Fig. 6: Photosynthetic electron transfer draws down the intracellular CO₂ concentration, relieving inhibition of LHCSR3 transcription.

a CO₂ concentration measured in the air stream coming out of the headspace of a column filled with 50 mL of HSM, sparged with air under HL. The two arrows in the graph indicate the addition of cells or DCMU. b WT cells were acclimated to LL HSM overnight shaken in flasks; the next day they were exposed to 300 µmol photons m⁻² s⁻¹ light in the presence or absence of 40 µM DCMU, shaken in flasks without or with sparging with VLCO₂. Samples were taken after 1 h. Presented are mRNA accumulation of LHCSR3, CAH4, LCIA. Data were normalized to LL (shown as dotted line in graph); n = 2 biological samples.