Fig. 4: Trojan BLI probes targeting diverse bacteria. | Nature Communications

Fig. 4: Trojan BLI probes targeting diverse bacteria.

From: In vivo bioluminescence imaging of natural bacteria within deep tissues via ATP-binding cassette sugar transporter

Fig. 4

a Confocal fluorescence images of MRSA or MDR E. coli after incubation with 0.06 mM GP-Si-BPs and corresponding flow cytometry analysis of uptake rates. b Confocal fluorescence images of MRSA or MDR E. coli after incubation with 0.06 mM GP-Si-Luc and corresponding flow cytometry analysis of uptake rates. c Confocal fluorescence images of MRSA or MDR E. coli after incubation with 0.06 mM GP-Si-Luc + GP-Si-BPs. d, e Confocal fluorescence images of bacteria mutants of ΔlamB and ΔmalE after incubation with 0.06 mM GP-Si-BPs (d) or GP-Si-Luc (e). The bacterial cell concentration is ~107 CFU. Scale bar, 25 μm. f Bioluminescence images of PBS buffer containing MRSA or MDR E. coli with different concentrations after incubation with 0.06 mM GP-Si-Luc + GP-Si-BPs. g Ex vivo bioluminescence imaging of human vitreous containing Enterococcus faecalis (1), Klebsiella pneumoniae (2), Streptococcus pneumoniae (3), Neisseria meningitidis (4), Haemophilus influenzae (5), Streptococcus pyogenes (6), Vancomycin (Van)-resistant Enterococcus (7), multidrug-resistant Staphylococcus aureus (MRSA) (8), Moraxella catarrhalis (9), or Salmonella para-typhi A (10) collected from patients with bacterial endophthalmitis. The vitreous without bacteria (0) from a healthy volunteer was set as the control. All imaging experiments were repeated three times with similar results.

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