Fig. 1: Extracellular action potential (EAP) recordings from in vivo epxeriments and biophysically realistic single-cell models.
From: Associations between in vitro, in vivo and in silico cell classes in mouse primary visual cortex
Extracellular action potential (EAP) recordings from in vivo experiments (a–d) and single-cell modeling (e–g). a Left, labels for Cre-line and morphology (spiny vs. aspiny) groups of single neurons used in this study characterized in vitro via intracellular electrophysiology and morphology reconstructions. Right, in vivo EAP waveform analysis typically results in two clusters, fast-spiking (FS) vs. regular-spiking (RS) units. b Primary visual cortex (V1) in the mouse brain (left) and typical cortical depth placement of a Neuropixels probe along V1 (right, from the Allen Reference Atlas-Mouse Brain86). c The 384 electrode sites of the Neuropixels probe are densely arranged along the linear shank probe (left; 20 μm vertical spacing, 2 sites per row; black squares: ___location of recording sites). EAP waveforms from two example units (unit #1: FS; unit #2: RS), including the channel with the largest amplitude (closest to the soma, bolded lines) and channels above and below the soma. d Top: the number of Neuropixels-implanted mice for wild-type (n = 24), parvalbumin-expressing (Pvalb, n = 8), and somatostatin-expressing (Sst, n = 12); bottom: the distribution of units per wild-type mouse recorded in V1 during drifting gratings (total number of units = 204). Distribution of units along the V1 depth axis with 0 indicating the center of layer 4. e Bio-realistic, single-cell models of V1 (“all-active”) are generated from in vitro experiments and activated via synaptic background to elicit intracellular activity and associated EAP signals in the vicinity of the cellular morphology. The cellular morphology is represented with a spherical soma and full dendritic reconstruction (axon not shown). Example simulations of EAP signals are shown for a spiny (top: red, cell ID: 395830185) and an aspiny (bottom: blue, cell ID: 469610831) single-cell model. f Four examples of the multi-channel EAP, including the channel with the largest amplitude (bolded lines, closest to the soma) and channels above and below the soma (top: 2 spiny models; bottom: 2 aspiny models). g In total, 33 single-cell models (15 spiny and 18 aspiny) were generated using a computational optimization framework and included in the study covering a range of major reporter lines and cortical depths. Source data are provided as a Source Data file.
