Fig. 6: Distinct cellular properties of multi-channel FS clusters.
From: Associations between in vitro, in vivo and in silico cell classes in mouse primary visual cortex
a The spike propagation symmetry index separates FS1 from FS2–3 (left, circles: experimental measurements; middle: effect size measured by Cohen’s d; right: mean spatiotemporal spike propagation of multi-channel clusters FS1–3; n = 130 FS1, n = 82 FS2, n = 69 FS3). Kruskal–Wallis H-test, F = 111.41, p-values corrected using the Holm–Bonferroni method for multiple tests, ***p < 0.001. Error bars represent a bootstrap 95% confidence interval. b Left: the multi-channel features (Vbelow, Vabove) clustering FS units (gray) and superposed multi-channel EAP features of models of Pvalb (blue, n = 9) and Sst (yellow, n = 9) neurons. Right: spike propagation symmetry index for Pvalb and Sst single-cell models. two-sample t-test, two-sided, p = 0.000998. c Pairwise comparison of model conductances between Pvalb and Sst models. The biggest and most statistically significant difference is shown in the dendritic Kv3.1 conductance. Mann–Whitney U test, two-sided, *p < 0.05. d Morphology bifurcation distance above (two-sample t-test, two-sided, p = 0.45) and below (two-sample t-test, two-sides, p = 0.03) soma between Pvalb (left, dark blue) and Sst (right, orange) models. *p < 0.05. e The inverse of spike propagation velocity vs. the bifurcation distance (line: linear fit; + indicates above soma, − indicates below soma). f Pairwise comparison of Pvalb vs. Sst model conductances (top panel, −log10(p-value), black line: p = 0.05; bottom panel, Cohen’s d effect size, black lines: |d| = 0.8). The comparison of dendritic Kv3.1 conductance is shown in (c). Mann–Whitney U test, two-sided, *p < 0.05. g Left: pairwise comparison between intrinsic properties of Pvalb and Sst neurons measured in vitro (same experiments as the ones used to develop single-cell models). Maximum spike rate to dc current injections separates between Pvalb and Sst neurons. Mann–Whitney U test, two-sided, p = 0.0067; Right: pairwise comparison between nine intrinsic properties of Pvalb vs. Sst neurons (top: statistical significance expressed in terms of −log10(p-value); solid line: p-value = 0.05, broken line: p-value = 0.01; bottom: Cohen’s d effect size, solid black line: |d| = 0.8) also used to generate the computational models. Mann–Whitney U test, two-sided, *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.
