Fig. 4: Trem2⁺ macrophages predominate within the mature myeloid fraction of mouse BCCs.

a UMAP plot of scRNA-Seq data of Cd45⁺ sorted cells from mouse BCC tumor (n = 1 primary tumor). b UMAP plot of scRNA-Seq data of myeloid cells from a. c UMAP plot of scRNA-Seq data of mature myeloid cells from b. d Heatmap showing the top 10 marker genes for the various clusters from c. e Stacked violin plot of various key myeloid-associated markers for each of the clusters from c. f Quantification of the flow cytometry confirmation analysis of the myeloid cells from mBCC. g Monocle analysis of the myeloid cells from c. h Diagram of M1 and M2 polarization experiments to generate M1 and M2 polarization scores. Top 20 differential genes between the M1- and M2-polarized cells were extracted and used for scoring. Feature plot of the M1- and M2-associated polarization scoring for clustering from c. i Spatial locations of Cd68⁺Trem2⁺ cells within mouse BCCs relative to the proliferative Ly6d^- tumor epithelium. RNAScope is for Cd68 (magenta), Trem2 (green), and protein staining is for K14-protein (white), Ki67-protein (red), and Ly6d-RNA (yellow). Red arrows show some Ki67⁺ cells. Green arrows some show Cd68⁺Trem2⁺ cells. Dotted yellow line indicates Ly6d⁺ region. Quantification of average cell distances of individual Cd68⁺Trem2⁺ cells. Scale bar = 50 μm. For f, i, n = 3 independent tumors. Error bars represent mean +/− SD. p values were calculated using an unpaired, two-tailed t test. For Source data are provided as a Source data file.