Fig. 8: Tumor assembly of the self-propagating myeloid niche.

a Diagram of transplantation strategies of male primary tumor into female NOD SCID mice. b RNAScope images of transplants showing male-specific Uty⁺ (red) F4/80⁺ (green) macrophages within the tumor. Yellow arrows point to F4/80⁺Uty⁺ cells. Image is representative of n = 2 independent tumors. c Diagram of transplantation strategies and the ability to trace SCAMs from primary tumor using the CD45.2 allelic variant. d Summary of the proportions for the CD45.2⁻ and Cd45.2⁺ from either Cd11b⁺ (left) or Cd11b⁺Trem2⁺ cells in the P0, P1, and P2 tumors. e Diagram summarizing self-propagating logic with approximate cell numbers. f Flow cytometry for Trem2 cells in the Cd11b⁺Cd45.2⁺ fractions from P0 and P1 tumors. g Quantification of the Trem2% of the Cd11b⁺Cd45.2⁺ and Cd11b⁺Cd45.2^- form the P0, P1, and P2 tumors. h Diagram of the transplantation strategy and subsequent sorting to isolate Cd45.2+ and CD45.2- cells from the P1 tumors for scRNA-Seq. i UMAP plots showing the different clusters from the Cd45.2+ and CD45.2- cells. j Feature plots showing key genes associated with SCAMs. k Violin plots showing key genes associated with SCAMs. l Diagram of the intratumor monocyte maturation injection strategy and subsequent analysis by flow cytometry. m Flow cytometry analysis of the injected GFP⁺ monocytes for SCAM-specific markers. n Quantification of the SCAM-specific markers Trem2 and Vcam1 (n = 4 independent injections into 4 different tumors). Length of each scale bar is noted in figure. For d, g, n = 4 tumors from P0, P1, and P2 allografts. Error bars represent mean +/− SD. p values were calculated using unpaired, two-tailed t test. Source data are provided as a Source data file.