Fig. 2: SYNCRIP plays a critical role in stress hematopoiesis.
A Immunoblots showing SYNCRIP KO in engrafted Syncrip Δ/Δ BM cells 3 weeks post pIpC injections. ACTIN as loading control. B Chimerism of donor-derived BM cells in recipient mice described in (A) at: pre-pIpC, 3-(p = 0.037), 8-(p = 0.0059), 16-(p = 0.0029) and 24-(p = 0.0041) weeks post pIpC (n = 3 donor, and n = 15 recipients/genotype at pre- and 3 weeks; n = 9 at 8-, 16- and 24- weeks). C Chimerism of donor-derived cells in stem/progenitor compartments of recipient mice described in (A) at 16 weeks post pIpC. LSK (p = 0.030), MP (p = 0.051), HSC (p = 0.0254), MPP1 (p = 0.0333), MPP2 (p = 0.0058), MPP4 (p = 0.141). D Quantitative summary of frequencies of HSC and MPPs cells within CD45.2+ LSK populations of recipient mice described in (A) at 16 weeks post pIpC. HSC (p = 0.95), MPP1 (p = 0.024), MPP2 (p = 0.037), MPP4 (p = 0.46). E Chimerism of donor-derived BM cells in secondary recipients at 8-, 16- and 24 weeks post-transplantation. (n = 3 donor, and n = 15 recipients/genotype). All p < 0.000001. F Chimerism of donor-derived cells in stem/progenitor compartments of secondary recipient mice (described in E) at 16 weeks post-transplantation (n = 3 donor, and n = 15 recipients/genotype). All p-values<0.000001. G Chimerism of donor-derived cells in total BM cells of secondary recipient mice at 1 week post-transplantation (n = 7 donor, n = 5 recipient/genotype). p-value=0.0256. H Kaplan–Meier analysis of survival of WT Syncripf/f and KO SyncripΔ/Δ mice following lethal irradiation (n = 5 each). p-value=0.0035. I, J Cell cycle analysis of (I) MPP1 (G0 p = 0.00089, G1 p = 0.15, S/G2/M p = 0.00095) and (J) HSCs Syncripf/f and SyncripΔ/Δ mice (n = 6 each genotype). Left: Representative flow plots. Right: Quantitative analysis. K Cumulative graphs tracking in vitro division of HSCs over the course of 60 h. L Representative flow plots showing gating strategy for CFSE stained HSC in recipient mice (described in G) at 1 week post-transplantation. M Quantitative summary of data shown in (L). Source data are provided as Source Data File. All data represent mean ± s.e.m. p values were calculated by two-tailed t test unless specified. *p < 0.05, **p < 0.01, ***p < 0.001 and ns not significant.
