Fig. 7: RNase J alters the growth and morphology of Mycobacterium smegmatis by affecting cell wall synthesis and lipid metabolism. | Nature Communications

Fig. 7: RNase J alters the growth and morphology of Mycobacterium smegmatis by affecting cell wall synthesis and lipid metabolism.

From: Structural insights into RNase J that plays an essential role in Mycobacterium tuberculosis RNA metabolism

Fig. 7

a The growth curve of wild-type (WT), RNase J KO, and RNase J complemented (COM) Msm strains. b The colony size difference among WT, KO, and COM Msm strains. c Cell lengths of WT, KO, and COM strains with transmission electron microscopy (TEM) observation. d Volcano plot of differentially expressed genes between WT RNase J and its KO strain by RNA-seq analysis. The significant differentially expressed genes were labeled by the standard of pval<0.05&|log2FC | >1, up as red, down as blue. e Heat map of significant differentially expressed genes between WT RNase J and its KO strain. The standard was shown as in (d). f The expression levels of twelve significant differential genes among the three strains were detected by the Q-PCR method. g Influence of rifampin on the mRNA stability of six represented genes in the WT or KO Msm strains. h, i The KEGG annotation analysis revealed that 49 genes in the metabolic pathway were significantly differentially expressed between WT RNase J and its knockout strain.

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