Fig. 3: Genetic interaction of SPL9 and miR172 with dwf5. | Nature Communications

Fig. 3: Genetic interaction of SPL9 and miR172 with dwf5.

From: Coordinated regulation of vegetative phase change by brassinosteroids and the age pathway in Arabidopsis

Fig. 3

a Phenotype of 25-day-old WT, dwf5, spl9-4, spl9-4 dwf5, pSPL9::rSPL9, pSPL9::rSPL9 dwf5, Ubi10::MIR172B, Ubi10::172B dwf5, toe1 toe2, and toe1 toe2 dwf5 plants grown in short days. Numbers indicate the first leaf with abaxial trichomes (n = 25 plants, ±SD). Different letters indicate significant difference between genotypes using one-way ANOVA at P < 0.01. Scale bar = 1 cm. b The percentage of leaf position with abaxial trichomes in pSPL9::rSPL9 and pSPL9::rSPL9 dwf5 (n = 90 plants, ±SD). c Abaxial trichome number on the first leaf of pSPL9::rSPL9 and pSPL9::rSPL9 dwf5. Asterisks denote significant difference from pSPL9::rSPL9 using two-tailed Student’s t-test at P < 0.001 (n = 10 plants, ±SD). d Leaf length/width ratio of the first leaf from 45-day-old WT, dwf5, pSPL9::rSPL9, and pSPL9::rSPL9 dwf5. Different letters indicate significant difference between genotypes using one-way ANOVA at P < 0.01 (n = 10 plants, ±SD). All experiments were repeated 3 times biologically.

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