Fig. 5: RpS3+/− cells exhibit higher levels of cell death during cell competition than in a non-mosaic context. | Nature Communications

Fig. 5: RpS3+/− cells exhibit higher levels of cell death during cell competition than in a non-mosaic context.

From: The PECAn image and statistical analysis pipeline identifies Minute cell competition genes and features

Fig. 5

a Wing discs carrying a mosaic anterior compartment (identified by the anterior fate marker, Ci, shown in white) containing competing RpS3+/− (red) and wildtype (unlabelled) cells and a posterior compartment (negative for Ci) that is entirely RpS3+/−. These samples were assessed for cell death via a staining for cleaved-Dcp-1 (green) and analyzed using PECAn. b PECAn output images identifying Minute cells in the anterior (orange) and posterior (cyan) compartments (left), the regions of the image positive for the Dcp-1 staining (middle) and the counts of individual Dcp-1-positive cells (right). c Output graphs and statistical tests generated by PECAn showing the density of Dcp-1-positive cells in the non-competing posterior compartment as compared to the RpS3+/− patch border in the anterior compartment for three separate experimental replicates, wherein each dot corresponds to an individual wing disc. Box and whisker plot denotes minimum, first quartile, median, third quartile, and maximum. Statistics reflect 2-sided Wilcoxon signed rank test with Cliff’s δ effect size metric. Biologically independent samples per replicate are as follows: replicate 1: n = 7; replicate 2: n = 9; replicate 3: n = 11. The scale bars correspond to 50 µm. Source data are provided as a Source Data file. Ci Cubitus interruptus, RFP = red fluorescent protein.

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