Fig. 2: NetBID2 identifies MYC as a hidden driver for KRAS-mutant lung adenocarcinoma (LUAD).

a Dataset and basic NetBID2 analysis workflow and oncoPrint of KRAS and MYC in LUAD from the TCGA data. b MYC activity inferred by NetBID2 (right) and its expression (left) in KRAS-mutant LUAD (n = 151 biologically independent samples) and wild-type normal samples (n = 59 biologically independent samples). The P values were estimated using two-tailed t test (getDE.limma.2G function in NetBID2). The center line represents the median and whiskers represents maximum (Q3 + 1.5*IQR) and minimum value (Q1 + 1.5*IQR). c The MYC transcription regulatory subnetwork in LUAD, inferred by SJARACNe from LUAD RNA-seq data. The orange and green edges correspond to positive and negative targets, respectively. The edge width is proportional to the mutual information of the interaction. d GSEA plot of the MYC-LUAD regulon for the differential expression in KRAS-mutant LUAD vs. wild-type normal samples. The P values were estimated using two-tailed t test (getDE.limma.2G function in NetBID2). e Function enrichment of the MYC target genes. Each box shaded in orange in the plot region indicates that the corresponding gene (column) is part of the pathway (row).