Fig. 2: Intra-tumoural heterogeneity of RMC. | Nature Communications

Fig. 2: Intra-tumoural heterogeneity of RMC.

From: SMARCB1 regulates a TFCP2L1-MYC transcriptional switch promoting renal medullary carcinoma transformation and ferroptosis resistance

Fig. 2

a Pseudo-bulk heatmap of expression of top markers of RMC and TAL clusters. b SWNE trajectory analysis of the naive RMC clusters using a set of selected markers per cluster and assuming TAL2 cells as origin. c UMAP representing the normalized merge of selected TAL, RMC, CAF and TAM clusters from the treated tumour (green hue) and the naive tumour (yellow hue). d Pseudo-bulk heatmap showing a set of known EMT markers in all RMC clusters. Note that as RMC4 were cycling RMC3 cells, they were omitted from the analysis to avoid redundancy. e GSVA analysis showing ontologies of indicated RMC clusters. f SWNE trajectory analysis of normalized merged RMC clusters from treated and naive tumours using a set of differentially expressed EMT markers. g SCENIC analysis of the treated tumour showing top regulons of RMC1/2 and TAL1 cells. Note that in brackets are indicated the number of genes (g) per selected regulon. h SCENIC analysis of the treated tumour indicating activities of TAL regulons and RMC1- or RMC2-specific regulons. i SCENIC analysis of the naive tumour revealing top regulons of RMC3/4 and TAL2/3 cells.

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