Fig. 5: REST could directly reveal versatile characteristics of dynamic nucleosomes.
From: A method for restoring signals and revealing individual macromolecule states in cryo-ET, REST
A The 2D slices of the raw tomogram (left) and REST-restored tomogram (right). The Fourier transforms are shown in the right corners. B Comparison of volumes denoised by the combination of Topaz-Denoise and IsoNet (T-I density, left) and REST-restored density (right). For clarity, each nucleosome core particle (cyan) or nucleosome with linker DNA (pink) contains two different views: the XY-view and the tilted view with the elongation (elongated view). C The 3D map of the reconstituted nucleosome with linker DNA by conventional single-particle reconstruction shows the asymmetric linker DNA.
