Fig. 2: The interaction between Bre1 and Rad51 promotes Rad51 loading, HR repair, and DNA damage response.

a Spotting assays showing the DNA damage sensitivity for indicated cells at indicated drug concentrations. The colorful dots represent different strains. b, c Microscopy analysis and quantification of cells with Rad52-YFP foci upon or after phleomycin treatment. Cells were treated with 20 µg/ml phleomycin for 2 h and then were released into media without the drug. Rad52-YFP foci were monitored at indicated time points. A representative image at 8 h is presented. Scale bar, 3 μm. Values represent the mean ± SD of three independent experiments (n = 3). d The survival rate for the WT or EK2A cells after short phleomycin treatment (20 µg/ml). Values represent the mean ± SD of three independent experiments (n = 3). e Scheme showing an ectopic recombination system. CO crossover, NCO non-crossover. f, h The survival rate of DSB repair by ectopic recombination for indicated cells. Values represent the mean ± SEM of three independent experiments (n = 3). g ChIP-qPCR analysis of Rad51-3xFLAG loading at 1 or 5 kb away from the HO cut site. Values represent the mean ± SEM of three independent experiments (n = 3). Samples were collected 4 h post galactose induction. Statistical analysis was calculated with the Student t-test (two-tailed). *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.