Fig. 4: Preparation and in vivo evaluation of the biomineralized microbial nanocomposite.
a TEM and size distribution images of Ads, OMVs@P2O-Ads, and CaP-OMVs@P2O-Ads. Scale bar=100 nm. b Energy spectrum analysis image of the biomineralized composite microbe. Scale bar=50 nm. This experiments in (a–c) were repeated three times independently with similar results. c In vivo fluorescence imaging of the multiple organs and tumors collected from the mice at 24 h post i.v. injection. From left to right: tumor, heart, liver, spleen, lung, and kidney. d Quantitation of the biodistribution of relative Ads contents in multiple organs and tumors after 24 h of different treatments by RT-qPCR. Data are presented as mean ± SD (n = 4 mice). *p < 0.05, **P < 0.01 and ****p < 0.0001 by two-tailed Student’s t-test. Source data are provided as a Source Data file. e Immunofluorescence images of LC3 autophagic proteins in tumor tissues. Blue represents DAPI-stained tumor cells and the green represents FITC-stained LC3 autophagic protein. Scale bar=2 mm. This experiment was repeated three times independently with similar results. f Quantitative analysis of fluorescence intensity. Data are presented as mean ± SD (n = 3 independent experiments). ****p < 0.0001 by two-tailed Student’s t-test. Source data are provided as a Source Data file. g The expression of autophagy-related protein LC3-I and LC3-II examined by western blot. This experiment was repeated three times independently with similar results. h Quantitation of relative Ads content in the tumor after 72 h of different treatments by RT-qPCR technique. Data are presented as mean ± SD (n = 3 independent experiments). N.S. (No Significance) P > 0.05 and ****p < 0.0001 by two-tailed Student’s t-test. Source data are provided as a Source Data file.
