Fig. 5: Temperature and feeding states are encoded in FLP neural pathway to control tonic speed elevation during glocal search.

Speed increase time course after starvation at 15 or 25 °C and in corresponding controls left on food (a); mean ± s.e.m. of n = 8, 6, 9, 6 assays for 15 °C Fed, 15 °C Starved, 25 °C Fed and 25 °C Starved, respectively. Speed measured after 6 h of starvation at 25 °C in wild type (N2), in transgenic lines with ablated neurons, or in animals carrying a Pmec-3::TeTx transgene blocking neurotransmission in FLP (b); mean ± s.e.m. (number of independent assays, n, at the bottom of each bar). FLP intracellular calcium levels at rest and following 2-min 10 °C thermal up- or down-steps (c–e); mean ± s.e.m. of n = 15, 13, 16, 17 assays for 15 °C Fed, 15 °C Starved, 25 °C Fed and 25 °C starved, respectively (c), resting calcium levels (d), corresponding to baseline period and temperature-evoked relative calcium changes (e), showing FLP encodes temperature and food signals as resting calcium levels without modulating the magnitude of thermal responses to short stimuli. Impact of temperature and food on speed elevations caused by a 5-min tonic optogenetic activation of FLP (f–h). Mean speed elevation profiles (±s.e.m. of n = 6 assays, each scoring ≥30 worms) with a first spike corresponding to an initial high reversal period (t = 60-90 s) and a second long-lasting speed elevation period (90-360 s) (f). Max (g) and average speed (h) during the long-lasting speed elevation period. *p < 0.05 and **p < 0.01 versus 15 °C Fed condition; #p < 0.05 and ##, p < 0.01 versus the indicated condition by Bonferroni posthoc tests (b, d, e, g, h). ns not significant. Source data are provided as a Source Data file.