Fig. 2: Coalescence of ER fragments (CERFs) in DKO adipocytes.
From: The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes
a Representative TEM images of P62 inclusions in DKO gonadal WAT (n = 4 mice) and BAT (n = 3 mice). #, P62 inclusion; LD, lipid droplet; M, mitochondria. b, c Representative EM images following immunogold co-labeling of P62 (pink dotted line) and the ER marker KDEL (green dotted line) in DKO gonadal WAT (n = 2 mice). Color-coded images, P26 (pink) and KDEL (blue). d–f Representative TEM images (d, e, n = 3-4 mice each) and TEM with immunogold labeling of the ER marker BiP (f, n = 3 mice) in DKO gonadal WAT or BAT. Arrows, membranes. g 3D reconstruction of a CERF in DKO gonadal WAT from 370 FIB-SEM images taken at every 10 nm (n = 2 independent repeats). h Diameters of the CERFs (n = 52) and P62 inclusions (n = 49) in DKO gonadal WAT based on TEM analysis of 4 mice with the mean of diameters indicated. i, j TEM images showing possible clustering (i) and fusion (j) of ER fragments in DKO BAT (n = 3 mice). Arrows, likely fused membranes inside CERFs. Source data are provided as a Source Data file.
