Fig. 2: Large orthogonal subsets of coiled-coils from the CCNG1 library. | Nature Communications

Fig. 2: Large orthogonal subsets of coiled-coils from the CCNG1 library.

From: A multiplexed bacterial two-hybrid for rapid characterization of protein–protein interactions and iterative protein design

Fig. 2

A Schematic of the CCNG1 Library design. All four-heptad coiled-coils with variation at the a-, e-, and g- positions were scored for interactions with the model of Potapov et al., and subsets of coiled-coils with large orthogonality gaps were identified. In total, we designed and tested 56 sets of orthogonal coiled-coils. B The orthogonal subset of coiled-coils with the largest number of on-target interactions (six on-target interactions). Grey boxes identify on-target interactions. C Number of interactions per orthogonal subset of coiled-coils. Dashed line represents the number of on-target orthogonal interactions in the CC0 library. Colours show the different backgrounds used, while the interfacial residues remained the same. Source data are provided as a Source Data file.

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