Fig. 2: Naïve OT-I cells display impaired crawling on inflamed pMBMECs upon recognition of cognate Ag on MHC class I under physiological flow in vitro.
From: Antigen recognition detains CD8+ T cells at the blood-brain barrier and contributes to its breakdown
A Graphs show the number of arrested naïve OT-I cells per FOV (872 × 654 μm) on TNF-α/IFN-γ stimulated WT pMBMECs without peptide (white bar), VSV peptide- (gray bar) and SIINFEKL (black bar) and on B2M-KO pMBMECs without peptide (light green bar), with VSV peptide- (green bar) and SIINFEKL peptide pulsing (teal color bar). Data were pooled from 3 independent experiments, analyzed using ordinary one-way ANOVA with Tukey’s multiple comparisons test, and shown as mean ± SD. See also Supplementary Movie 2. B Quantification of post-arrest behavior of naïve OT-I cells on WT and B2M-KO pMBMECs during 30 min recording. The number of arrested naïve OT-I T cells for each condition was set to 100% and the behavioral categories are shown as fraction thereof. Data is shown as mean ± SD from 3 experiments. See also Supplementary Movie 2. Violin plots of the crawling distance in μm (C) and speed in μm/min (D) of naïve OT-I cells on TNF-α/IFN-γ -stimulated WT and B2M-KO pMBMECs are shown. Values are pooled from three individual expeirments and shown as mean ± SD. For each condition 4–18 cells were tracked. Data were analyzed using ordinary one-way ANOVA with Tukey’s multiple comparisons test. Source data from (A, B, C and D) are provided as a Source Data file.
