Fig. 4: PIE-PUM2 uncovers mRNA targets in single cells.
From: PIE-seq: identifying RNA-binding protein targets by dual RNA-deaminase editing and sequencing
a The workflow of PIE-Seq for 10-cell and single-cell samples. b IGV browser tracks of the CDKN1B gene showing PIE-PUM2 editing sites in single-cell and ten-cell samples. C-to-U editing rates over 10% are indicated as blue (Cytosine) and red (Uracil). c Venn diagrams showing PUM2 targets identified in single-cell and ten-cell samples (25% down-sampling, details in supplementary Fig. 4c–e) largely overlapped with those of bulk PIE-PUM2.
