Fig. 5: Applying PIE-PUM2 to cortical progenitors and neurons identifies App as one of its targets.
From: PIE-seq: identifying RNA-binding protein targets by dual RNA-deaminase editing and sequencing
a The IUE and PIE-Seq workflow in the developing mouse neocortex. b Immunostaining results showing the distribution of EGFP-positive cells in the E15.5 dorsal cortex. The germinal zone is indicated by anti-PAX6 (red). Hoechst (blue) labels DNA. Scale bar, 200 μm. Representative image from three independent experiments. c Venn diagrams showing PUM2 target genes in neurons and NPCs. d RNA-Seq and iCLIP tracks of the App gene showing the NPC-specific PIE-PUM2 target site and Pum2 iCLIP signals from newborn mouse brains, respectively. e Differential gene expression analysis showing comparable App mRNA expression levels in NPC and Neuron groups. Each group with n = 3 biologically independent samples, p value from the Empirical Bayes method in limma. Data were presented as mean values ± SD. Source data are provided as a Source Data file. f Immunostaining results showing Pum2 (red) and App (green) expression in the E14.5 dorsal forebrain. Hoechst (blue) indicates genomic DNA. The abbreviations VZ, IZ, and CP denote the ventricular zone, intermediate zone, and cortical plate, respectively. Scale bar, 100 μm. Representative images from two independent experiments.
