Fig. 2: HIV-1 dependence on Nup35, Nup153, and POM121 is regulated by CypA.
From: The HIV-1 capsid core is an opportunistic nuclear import receptor
a HIV-1, SIV, FIV, and MLV infection in the absence or presence of CsA in Nup35-depleted cells. WT HIV-1 or CA-mutant HIV-1 or various retroviruses infection of control or target siRNA transfected HeLa cells in the presence or absence of cyclosporin (CsA). n = 2 technical replicates, representative of two independent experiments. Western blotting confirmed knockdown efficiency. b CsA restores HIV-1 infection in Nup35-depleted MT4 cells. WT HIV-1 or CA-mutant HIV-1 infection of control or target shRNA transduced MT4 cells in the presence or absence of CsA. n = 2 technical replicates, representative of two independent experiments. Western blot analysis confirmed Nup35 depletion by three different shRNAs. c CsA restores A92E and G94D HIV-1 infectivity in Nup35 knockdown cells. WT HIV-1 or CA-mutant HIV-1 or HIV-1 or MLV infection of control or Nup35 siRNA transfected HeLa cells in the presence or absence of CsA. n = 2 technical replicates, representative of three independent experiments. d CypA depletion by siRNA restores HIV-1 infectivity in Nup35 and POM121 knockdown HeLa cells. WT HIV-1 or CA-mutant HIV-1 infection of control or single-knocked down or double-knocked down HeLa cells. n = 2 technical replicates, representative of three independent experiments. Source data are provided as a Source Data file.
