Fig. 4: Transplantation of microbiota from fluctuated temperature-treated mice dampens muscle function.
From: Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota
a–c Determination of exercise capacity by conducting rotarod test (a), grip test (b), and treadmill test (c) in FMTrt, FMTlt, and FMTft mice. n = 6 biologically independent animals per group. d Weight of dissected quadriceps femoris, tibialis anterior, gastrocnemius, and soleus muscles. n = 5 biologically independent animals per group. e–g Representative images of HE staining of cross-section of gastrocnemius muscle (e), quantification of mean cross-sectional area (f), and distribution of muscle fibers (g). Bar = 80 μm. n = 5 biologically independent animals per group. h–i Representative images of succinate dehydrogenase (SDH) staining of cross-section of tibialis anterior (h) and quantification (i) of stained muscle fibers. Bar = 125 μm. n = 5 biologically independent animals per group. j Expression levels of Myostatin, Atrogin1, Musa, and MuRF1 measured by qPCR. n = 5 biologically independent animals per group. Data presented are mean±s.e.m. One-way ANOVA test for multiple comparisons with Tukey’s test for post hoc corrections. Source data are provided as a Source Data File.
