Fig. 4: Effects of the CscB transporter on sucrose and glucose secretion of PCC 7942 derived strains. | Nature Communications

Fig. 4: Effects of the CscB transporter on sucrose and glucose secretion of PCC 7942 derived strains.

From: Unlocking the potentials of cyanobacterial photosynthesis for directly converting carbon dioxide into glucose

Fig. 4

a Engineering strategies to facilitate sucrose secretion in PCC 7942. b Sucrose/glucose secretion in SZ130 (Δglk1-Δglk2-ΔinvA) and SZ153 (Δglk1-Δglk2-ΔinvA-ΔNS3::cscB) with or without IPTG induction. c Extracellular glucose and sucrose accumulations of FL92 (ΔNS3::cscB), SZ21 (ΔinvA-ΔNS3::cscB), and SZ153 (Δglk1-Δglk2-ΔinvA-ΔNS3::cscB) with or without IPTG induction. The cells were grown in BG11 for 8 d. Data are presented as mean values ± SD. All sample size n (biological replicates) is 6. Glk√ and Glk×: intact and deficient glucokinase, respectively; IPTG√: strain grown in the presence of 0.1 mM IPTG; IPTG×: no IPTG added to the BG11 medium; CscB×: no CscB in the strain; CscB√: strain expressing CscB; Invertase √ and Invertase×: intact and deficient invertase, respectively; CBB cycle Calvin–Benson–Bassham cycle, Fru-1,6-bisP fructose-1,6-bisphosphate, Fru-6-P fructose-6-phosphate, UDP-Glc UDP-glucose, Suc-6-P sucrose-6-phosphate, Glc-6-P glucose-6-phosphate, Glc-1-P glucose-1-phosphate, Sps sucrose-phosphate synthase, Spp sucrose-phosphate phosphatase, Fk fructokinase, CscB sucrose permease, Pgi glucose-6-phosphate isomerase, Pgm phosphoglucomutase, Fbp fructose-1,6-bisphosphate I, Glpx fructose-1,6-bisphosphatase II/sedoheptulose-1,7-bisphosphatase, Galu UTP-glucose-1-phosphate uridylyltransferase. Source data are provided as a Source Data file.

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