Fig. 5: Effects of synpcc7942_1161- G274A mutation on glucose secretion of PCC 7942 strains. | Nature Communications

Fig. 5: Effects of synpcc7942_1161- G274A mutation on glucose secretion of PCC 7942 strains.

From: Unlocking the potentials of cyanobacterial photosynthesis for directly converting carbon dioxide into glucose

Fig. 5

a Sanger DNA sequencing of the amplified synpcc7942_1161 gene region of SZ3 and SZ17. b Effect of synpcc7942_1161 gene deficiency on glucose secretion of SZ3 strain. c The construction strategy of the SZ182 strain by introducing the synpcc7942_1161- G274A mutation into the genome of PCC 7942; SZ181 carrying the same inserted antibiotics resistance marker was used as a control strain. The numbers indicate the elevated folds of respective transcript abundances in SZ182 compared to SZ181. d Overexpression effect of synpcc7942_1161 (SZ192) on glucose production of the SZ17 strain. e The construction strategy to replace the galP gene in SZ17 with synpcc7942_1161 (with or without G274A mutation). f Comparisons of the effects of synpcc7942_1161 and synpcc7942_1161-G274A on promoting glucose secretion when used for replacing GalP on the chromosome of SZ17. Data are presented as mean values ± SD. All sample size n (biological replicates) in (b, d and f) is 3. All sample size n (biological replicates) in (c) is 6. Source data are provided as a Source Data file.

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