Fig. 2: Evaluation of quality of targeted data generated using hybrid-DIA. | Nature Communications

Fig. 2: Evaluation of quality of targeted data generated using hybrid-DIA.

From: Hybrid-DIA: intelligent data acquisition integrates targeted and discovery proteomics to analyze phospho-signaling in single spheroids

Fig. 2

Demonstration on the accuracy, precision, and linearity of the quantification in the MSx scans using the Pierce Suitability Standard Mix, which is comprised of 7 peptides, each one with 5 isotopologue sequences present in a dilution series ranging from 0.5 pmol/µl to 0.3 fmol/µl. The isotopologue with the highest concentration (Heavy4) was used as a triggering peptide, and the subsequent peptides were used as endogenous counterparts, and were triggered sequentially in the acquisition cycle. RT: retention time. Source data are provided as a Source Data file.

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