Fig. 3: Profiling of intratumoral T and myeloid cells.

A Percentage of TILs, assessed by H&E staining, in PF vs PD. B Representative Multiplex Immunohistochemistry (mIHC) staining of the tumor microenvironment (TME) for CD3⁺, Granzyme B (GZMB⁺), FOXP3⁺, CD11c⁺, Pan-cytokeratin (Pan-CK) tumor cells and PD-L1⁺ cells in PF vs PD. C Number of CD3⁺, GZMB⁺, FOXP3⁺, CD11c⁺ cells/mm² in the S vs T areas in PF vs PD. D Number of PD-L1⁺ Pan-CK^- cells/mm² (i.e., non-tumor cells) in the S vs T areas (left), and of PD-L1⁺ Pan-CK⁺ cells in the T area in PF vs PD. E Representative mIHC staining of the TME for GZMB⁺, FOXP3⁺, CD11c⁺, Pan-CK⁺ tumor cells, and Ki67⁺ cells in PF vs PD. F Number of proliferating (Ki67⁺) GZMB⁺ (left) and FOXP3⁺ (right) cells/mm² in the S vs T areas, in PF vs PD. G Representative mIHC staining of the TME for CD8⁺, CD28⁺, PD^-1⁺, CD11c⁺, CD86⁺, and Pan-CK⁺ tumor cells in PF vs PD. H Number of CD11c⁺CD86⁺ double positive antigen-presenting cells/mm² (APC) (left) and CD8⁺CD28⁺ cells/mm² (right) in the S vs T areas, in PF vs PD. I Representative mIHC staining (from a PD) highlighting CD3⁺ cells (yellow dots) and PD-L1⁺ Pan-CK⁺ tumor cells (red dots). The white lines represent the intercellular distance between each CD3⁺ and PD-L1⁺ tumor cell in proximity. J Cumulative data of the proximity of CD3⁺ cells to PD-L1⁺ tumor cells in the S and T areas of PF vs PD. K Cumulative data of the proximity of FOXP3⁺ Ki67⁺ cells to CD11c⁺ Ki67⁺ cells in the S area of PF vs PD. L Cumulative data of the proximity of CD8⁺ CD28⁺ cells to CD11c⁺ CD86⁺ APC (left) and to Pan-CK⁺ cells (right) in the S area of PF vs PD. In all panels, n = 11 for PF and n = 4 for PD (biologically independent samples). Panels B, E, G, and I are representative of 15 biologically independent samples. Data were presented as individual values (mean ± SEM). Statistical tests: two-tailed unpaired and paired t-tests (Mann–Whitney and Wilcoxon matched-pairs signed rank test) in all panels. T tumor, S Stroma. Source data are provided as Source Data File.