Fig. 5: Bioactive sphingolipids phytosphingosine and phytoceramide accumulated in csg2Δ cells.
From: The ER calcium channel Csg2 integrates sphingolipid metabolism with autophagy
a, b Phytosphingosine and ceramide accumulated in csg2Δ cells after starvation. 100OD WT cells and csg2Δ cells were collected after 3 h of starvation in SD-N medium and subject to lipidomics analysis. Experiments were set up in three biologically independent samples (a). The mostly increased lipids with high confidences were found to be bioactive sphingolipids, phytoceramides (PhytoCer) (colored in red cycles), and phytosphingosine (PHS) (colored in red square). Analyzed with t-test, n = 3 biologically independent samples. c The ratios of lipids that were obviously increased in Csg2 deleted cells. d Brief schematic diagram of the process of sphingolipid synthesis in yeast.
