Fig. 5: Anti-virulence analysis of BHA against F. graminearum, F. oxysporum, R. solani, and P. striiformis f. sp. tritici.
From: Inhibition of chitin deacetylases to attenuate plant fungal diseases
a Phylogenetic analysis of pathogenic fungal CE4 deacetylases. b Conservation of the catalytic domains of VdPDA1, FgCDA, FoPDA1, Pst_13661, and RsCDA. SP represents the signal peptide. c Highly conserved residues around the active site of VdPDA1, based on ConSurf analysis of 247 phytopathogenic fungal CDA sequences. The surface is colored by the ConSurf score according to the indicated scoring scheme. d–f Inhibition constant Ki values of BHA against FgCDA (d), FoPDA1 (e), and RsCDA (f). g Scheme of the anti-virulence analysis method and disease phenotypes of soybean hypocotyls infected with the indicated pathogens. h Fungal biomass, as determined by qRT-PCR, of soybean hypocotyls infected with the indicated pathogens with BHA or ddH2O (the solvent) pre-treatment. Bars represent mean ± SEM (n = 3). Statistical significance was determined by Student’s two-sided unpaired t test. Source data are provided as a Source Data file. i Disease indices of wheat plants infected with P. striiformis f. sp. tritici at 19, 21, and 23 dpi, which were pre-treated with varying doses of BHA. Bars represent mean ± SEM (n = 7). Statistical significance was determined by Student’s two-sided unpaired t-test. Source data are provided as a Source Data file.
