Fig. 2: ExPRESSO is compatible with mass spectrometry-based highly-multiplex imaging technologies.
From: Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology
a Comparison of MIBI (top) and ExPRESSO-MIBI (bottom) imaging of two sections of a human tonsil with markers for helper T cells (CD3 and CD4), cytotoxic T cells (CD3 and CD8), B cells (CD20 and Pax-5), B cells and follicular dendritic cells (CD21), antigen-presenting cells (HLA-DR), macrophages (CD68), structural intermediate filaments (Vimentin), proliferation (Ki-67), nuclear envelope (Lamin A/C), and nuclei (Histone H3). The four images on the right are enlarged views of the image on the left. Scale bars (the ExPRESSO-MIBI scale bars indicate the pre-expansion dimensions based on a 3.7-fold expansion): 50 μm (left), 10 μm (right). b Comparison of IMC (left and middle) and ExPRESSO-IMC (right) imaging in two adjacent sections of a human tonsil with markers for T cells (CD3), B cells (CD20), B cells and follicular dendritic cells (CD21), structural intermediate filaments (Vimentin), proliferation (Ki-67), nuclear envelope (Lamin A/C), and nuclei (Histone H3). The middle image is an enlarged view of the image on the left, and its size was matched with the right image. Enlarged views are shown at the top left in the middle and right images. Scale bars (the ExPRESSO-IMC scale bars indicate the pre-expansion dimensions based on a 3.7-fold expansion): 100 μm (main), 25 μm (middle and right, enlarged).
