Fig. 3: Elevating local ApoA1 concentrations enable tumor control in a macrophage–T-cell immune-codependent manner.

a GEPIA2 expression analysis of APOA1 and its receptors. Data match the TCGA-GBM and GTEx datasets. Data are presented as median with interquartile range. Log₂FC = 1. Statistical significance was determined using one-way ANOVA with Tukey test, P value = 0.05. b Protein expression analysis of APOA1 in GBM cell lines cultured in vitro. n = 2 independent experiments. c Representative flow plots of APOA1 receptor ABCA1/G1 expression levels in GBM cell lines cultured in vitro. The FCM plots are representative of 3 independent experiments. d Cell proliferation assay for ApoA1-treated GBM cells. GBM cells (5 × 10³) were seeded in 96-well plates and cultured with either vehicle or ApoA1 (10 µg/ml) in 1% FBS medium for 0–4 days. Cell proliferation kinetics were identified by MTT assay. n = 3 independent experiments. e Kaplan–Meier survival curves of intracranial GBM^Puro- or GBM^APOA1-bearing immunodeficient and immunocompetent mice. GL261 in Nude, n = 6 mice per group. GL261 in C57, n = 9 mice per group. G422 in Nude and KM, n = 7 mice per group. f Quantification of interstitial fluid cholesterol content in APOA1-expressing intracranial GBM tumor tissues. n = 5 mice per group. g Experimental setup, validation and Kaplan–Meier survival curves of intracranial GL261^APOA1-bearing C57BL/6J mice with depletion of immune cells. n = 6 mice per group. h Determination of TAM-T-cell codependency in tumor killing. Data are from 3 independent experiments, and each point represents the mean of triplicate wells. Statistical significance was determined using the log-rank test in e, g, two-way ANOVA with Sidak’s test in d, the Mann–Whitney test (Two-tailed) in f, or the unpaired t test (Two-tailed) in h. Data shown are the mean ± SD. Source data are provided in the Source Data file.