Fig. 5: Ultrafast absorption and fluorescence spectroscopic analysis of CpcL-PBS. | Nature Communications

Fig. 5: Ultrafast absorption and fluorescence spectroscopic analysis of CpcL-PBS.

From: Cryo-EM and femtosecond spectroscopic studies provide mechanistic insight into the energy transfer in CpcL-phycobilisomes

Fig. 5

a Femtosecond time-resolved transient absorption spectra between 550 nm and 700 nm. The excitation wavelength is set at 590 nm and duration of the excitation laser pulse is 100 fs. Six time-resolved spectra of 100 fs, 1 ps, 10 ps, 20 ps, 200 ps, and >1200 ps are shown. b Four species-associated absorption spectra (SAS) are obtained from global analysis of the transient absorption spectra: P1 (3.6 ps), P2, (40 ps), RS (200 ps), RL (200 ps), and T (>1200 ps) and their bleaching is at 631 nm, 637 nm, 644 nm, 668, and 669 nm, respectively. c Kinetics of the four corresponding species-associated components. d Dynamics-associated difference fluorescence spectra (DAS) of CpcL-PBS excited at 565 nm; fluorescence is recorded with a streak camera. e Species-associated emission spectra (SAS) of CpcL-PBS. Peak wavelengths of each component are shown. f Kinetics of the three corresponding species-associated decay components.

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