Fig. 4: Nuclear magnetic resonance structure analysis of Cytc mutants.

A Chemical shift perturbation (CSP) bar plot of Cytc backbone amide signals (ΔCSPX = CSPX - CSPWT) of reduced Cytc mutants (K39E in pink, K39Q in yellow, and K39R in purple). A scheme of the secondary structure elements is included at the top and the mutated position is indicated with dashed lines. B Surface maps of NMR ΔCSP of reduced Cytc mutants. Each map shows residues colored according to ΔCSP values calculated previously. The Heme group is in green and non-assigned and proline residues are in tan. Source data are provided as a Source Data file.