Fig. 1: The enzyme mining process for identifying BHETase. | Nature Communications

Fig. 1: The enzyme mining process for identifying BHETase.

From: Discovery and mechanism-guided engineering of BHET hydrolases for improved PET recycling and upcycling

Fig. 1

a Except for BHETase, both PETase, and MHETase have been well studied. PET: polyethylene terephthalate; TPA: terephthalic acid; EG: ethylene glycol; BHET: bis-2-hydroxyethyl terephthalate; MHET: mono-2-hydroxyethyl terephthalate. b An overview of the BHETase mining process to screen and identify microbes and enzymes with PET degradability. Icon graphics of this figure was created by BioRender.com. c The obtained 70 microbes with degrading PET film after multi-round separation and isolation. Reaction conditions: pH 7.5, 30 °C, 2 g/L PET powder, and 5% inoculum for 5 days. All measurements were conducted in triplicate (n = 3), and the mean values were used for generating the heat maps. d The overview of Chryseobacterium sp. PET-29 (No. 29) and e Bacillus subtilis PET-86 (No. 70) were cultivated in LB agar plates, salt agar plates with PET powder, and olive oil plates, respectively. The scanning electron microscopy (SEM) images of Bacillus subtilis PET-86 (No. 70) and Chryseobacterium sp. PET-29 (No. 29) cells were grown on PET powder. Results were reproduced three times independently; representative micrographs are shown.

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