Fig. 6: Closed-loop recycling and open-loop PET upcycling based on the BHETase-joined tandem chemical and biological PET degradation system. | Nature Communications

Fig. 6: Closed-loop recycling and open-loop PET upcycling based on the BHETase-joined tandem chemical and biological PET degradation system.

From: Discovery and mechanism-guided engineering of BHET hydrolases for improved PET recycling and upcycling

Fig. 6

a The schematic of the closed-loop PET recycling and open-loop PET upcycling pathways. TEBAC: benzyl triethylammonium chloride. Icon graphics of this figure was created by BioRender.com. b The schematic of the closed-loop PET recycling. c The schematic of the open-loop PET upcycling. d Theoretical maximum synthesis yield of p-phthaloyl chloride from 21 commercial pc-products. Chemical glycolysis conditions: glycolysis of 5 g PET, 3 h of reaction time, 200 °C of temperature, and 0.1 g of potassium carbonate loading. Enzymatic catalysis condition: 2 mL of 0.5 mg/mL ΔBsEst and ΔChryBHETase hydrolyzing the BHET obtained from PET glycolysis at 30 °C within 24 h, respectively. Error bars correspond to the standard deviation (s.d.) of three measurements (n=3).

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