Fig. 1: Strain isolation procedure.
From: Identification of a deep-branching thermophilic clade sheds light on early bacterial evolution
a Location of L-vent at the 9°N vent field on the East Pacific Rise. The map was generated by Ocean Data View (Schlitzer, Reiner, Ocean Data View, https://odv.awi.de, 2021). b The black L-vent chimney sample collected in an anaerobic tube. c Schematic diagram of the “Subtraction-Suboptimal” (StS) strategy used to isolate Zhurongbacter thermophilus strain 3DAC in this study. d The track of proportion changes of 3DAC closely related sequences in the chimney sample, original enrichment, and different transfers. Labels: Chimney, the chimney sample; CH8-50C-S0, original enrichment; CH8-50C-S1 to -S5, the first to fifth subcultures transferred from the original enrichment. All samples indicate that strain 3DAC represents a rare species. e Transmission electron micrograph of 3DAC, in which 3DAC cells form long filaments with chains of up to 11 cells or more; the micrograph showing a connection between two 3DAC cells; and a long lateral flagellum of 3DAC. The TEM experiment was repeated four times, and long filamentous patterns were observed each time. Source data are provided as a Source Data file.
