Fig. 6: Analysis of procyanidin dimers and trimers in maize seeds expressing GmANR1 or SbTT2. | Nature Communications

Fig. 6: Analysis of procyanidin dimers and trimers in maize seeds expressing GmANR1 or SbTT2.

From: An unconventional proanthocyanidin pathway in maize

Fig. 6

Selected ion chromatograms of procyanidin dimers in standards and PAs extracted from seeds of untransformed and transgenic maize ST (a), OSA (b), and B104 (c). d Selected ion chromatograms of procyanidin trimers in PAs extracted from seeds of Medicago (Mt), soybean (Gm), untransformed ST maize, and ST maize expressing GmANR1. SD, chemical standards; ST, Suntava maize; ST-GmANR1, transgenic maize seeds obtained from crosses between HiII-GmANR1 and ST; OSA, Osage maize; OSA-GmANR1, transgenic maize seeds obtained from crosses between HiII-GmANR1 and OSA; B1-B4, procyanidin dimers B1-B4 (m/z = 577.1360 ± 10 ppm); iso-B2, procyanidin B2 isomer with (+)-epicatechin as the starter unit; iso-B4, procyanidin B4 isomer with (+)-epicatechin as the starter unit; C1, procyanidin C1 trimer (m/z = 865.1981 ± 10 ppm).

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