Fig. 4: Growth variation in the premolar region. | Nature Communications

Fig. 4: Growth variation in the premolar region.

From: Bat teeth illuminate the diversification of mammalian tooth classes

Fig. 4

a Growth rate between the Canine (C) and the P4 position during development for species in our four groups calculated from the µCT scans. b, c Cell division patterns in the premolar and molar regions at relevant stages for Glossophaga soricina (GS, long jaw), Monophyllus redmani (MR, long jaw), Mormoops blainvillei (MB, regular jaw), Erophylla sezekorni (ES, 2P3MR) and Artibeus. jamaicensis (AJ, short jaw). To visualized cell division patterns during the development of the bats, pregnant females have been injected with EdU which is then visualized in whole mount dissected jaw (experiment performed three times with two replicates) (b). Alternatively, dividing cells were labeled using an antibody against PCNA in cryosections (experiment performed three times with two replicates) (c). The position of the canine is indicated with a white dotted line. CS: Carnegie Stage. Scale bar: 200 µm. d Model of the dynamics of emergence of premolar and molar buds and differential growth, based on µCT-scan measurements (a) and cell division experiments (b, c). Dental lamina develops in both directions from the initial dP4 and M1 tooth buds independently for premolars and molars. Differential growth rate for the different groups is indicated in green. Source data for (a) are available as Source Data file 3.

Source Data file 3.

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