Fig. 2: Tripartite co-culture design enables de novo eugenol biosynthesis.
From: A tripartite microbial co-culture system for de novo biosynthesis of diverse plant phenylpropanoids
A Consolidated bioconversion strain design containing endogenous tyrosine pathway modifications and 8 heterologous enzymes for de novo eugenol production in a single strain. B Tripartite co-culture design in which Modules I-III were separated into three distinct strains to minimize toxic side product buildup and appropriately distribute metabolic burden. C Representative high-performance liquid chromatography (HPLC) analysis results comparing consolidated bioconversion (unsuccessful) and tripartite co-culture (successful) strategies for eugenol production, with comparison to an analytical standard, as well as negative control (wild type BL21(DE3) E. coli). The expected product ___location is represented by a vertical dashed line.
