Fig. 4: Pathway optimization maximizes de novo eugenol production.
From: A tripartite microbial co-culture system for de novo biosynthesis of diverse plant phenylpropanoids
A Production of eugenol and key intermediates via full Module III (SMB150) as well as ferulic acid to coniferyl alcohol (SMB151) and coniferyl alcohol to eugenol (SMB146) portions. Table containing molar conversion of 2 mM substrates after 48 h is included above graph. B Heat map of eugenol titer achieved across a range of Module II: Module III ratios (Module I ratio held at 1). C Eugenol titer achieved by optimized tripartite co-culture at 1 ml deep well scale, demonstrating minimal accumulation of hydroxycinnamic acid side products. D Eugenol titer of tripartite co-culture at 30 ml flask scale, demonstrating first and highest de novo phenylpropene bioproduction. Data are mean ± SD.; n = 3 biological replicates. Source data are provided as a Source Data file.
