Fig. 3: Sequence and structure comparison between mtRNASer(UGA) and mtRNASer(GCU). | Nature Communications

Fig. 3: Sequence and structure comparison between mtRNASer(UGA) and mtRNASer(GCU).

From: Structural basis for a degenerate tRNA identity code and the evolution of bimodal specificity in human mitochondrial tRNA recognition

Fig. 3

a Sequence conservation in mtRNASer(UGA) (left) and mtRNASer(GCU) (right) across 41 primate species plotted onto the secondary structures of human mtRNASer variants. Conservation scores reach from 100% (red) to <50% (teal). b Sequence conservation between primate mtRNASer(UGA) and mtRNASer(GCU) variants. Yellow indicates nucleotides present only in mtRNASer(UGA), orange indicates nucleotides present only in mtRNASer(GCU). Nucleotide identities are given if positions are >90% conserved and shared between both human tRNASer isoacceptors. Green lines indicate the interfaces of both tRNAs with mSerRS. c Superposition of the tertiary structures of mtRNASer(UGA) (green) and mtRNASer(GCU) (tomato; PDB 7U2A)35. The superposition is based on the acceptor-stem-T-stem regions of both tRNAs. The region enlarged in d is indicated. d Close-up view of the superimposed elbow regions from mtRNASer(UGA) (green) and mtRNASer(GCU) (tomato), showing the shift and rotation of A56 in mtRNASer(GCU) relative to the homologous C56 in mtRNASer(UGA) as a consequence of the additional T-stem pair U53A:A60A (see also b). Dashed lines indicate the H-bonds of the tertiary G19:C56 base pair that is found in all canonical tRNAs, but absent from mtRNASer(GCU).

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