Fig. 8: EBF2/3 Show species-specific biases in mesenchymal cell subpopulations.

a Co-projected UMAPs of CS17 and E12.5 craniofacial tissues of all cell types. Expression plots of EBF2 (orange) and EBF3 (blue) in human and mouse demonstrate highly different rates of coexpression (pink) based on species (23.49 human, 9.31 mouse). b Co-projected UMAPs of mesenchymal subclustering of CS17 and E12.5 craniofacial tissues. Expression plots of EBF2 (orange) and EBF3 (blue) in human and mouse demonstrate highly different rates of coexpression (pink) based on species (25.28 human, 10.88 mouse). c Expression of EBF3 in mesenchymal subclusters shown in (b), split by species. Clusters 0, 1, and 6 demonstrate human-biased expression, while clusters 4 and 5 demonstrate mouse-biased expression. EBF2 expression in mesenchymal subclusters displays human-biased expression in most clusters, with only cluster 6 showing bias towards mouse (***p < 0.05 for clusters with median expression >1 in at least one species based on Wald test performed by DESeq2¹⁴¹, pink stars indicate human bias, blue stars indicate mouse bias).