Fig. 1: The dynamic gene expression upon persistent flg22 treatment as analyzed by RNA-seq. | Nature Communications

Fig. 1: The dynamic gene expression upon persistent flg22 treatment as analyzed by RNA-seq.

From: BIK1 protein homeostasis is maintained by the interplay of different ubiquitin ligases in immune signaling

Fig. 1

a The deferentially expressed genes (DEGs) were grouped into 13 clusters. Twelve-day-old Arabidopsis seedlings were treated with 5 µM flg22 for the indicated times. Totally 13409 DEGs (log2[fold change, FC] > 1, Padj < 0.05) were grouped via K-means clustering by STEM software. The black number indicates the cluster ID, and the brown number shows the number of genes in each cluster. The gray lines show the dynamic gene expression profile during the 8 h time course of flg22 treatment, and the centroid gene expression profile was plotted by the dark black lines. The FPKM value for each time point was the mean of three biological replicates. b Four gene expression patterns (#1–4) during the 8 h time course of flg22 treatment. c The expression heatmap of immune related genes with the expression pattern #2, based on the FPKM values of three biological replicates. d GO enrichment analysis of DEGs with the expression pattern #2, GO terms were determined using the agriGo database. The top 10 biology process (BP) terms were shown.

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