Fig. 4: Catalytic activity of targeted CYP76AK subfamily members with two substrates. | Nature Communications

Fig. 4: Catalytic activity of targeted CYP76AK subfamily members with two substrates.

From: Functional divergence of CYP76AKs shapes the chemodiversity of abietane-type diterpenoids in genus Salvia

Fig. 4

a Extracted ion chromatogram (EIC) analysis of the CYP76AK catalytic reaction products in vitro with 11-hydroxyferruginol. To obtain 11-hydroxyferruginol, all plasmids contained SmCYP76AH3, which converts ferruginol to 11-hydroxyferruginol. A sample with only SmCYP76AH3 protein functioned as the negative control. Peaks corresponding to potential products are indicated by the m/z value of 11, 20-dihydroxyferruginol (m/z 317.2115), and carnosic acid (m/z 331.1900). b EIC overlays showing in vitro catalytic activity of CYP76AKs with 11-hydroxysugiol. The sample with the empty vector (pESC-His) functioned as the negative control. Peaks corresponding to potential products are indicated by the m/z value of 11, 20-dihydroxysugiol (m/z 331.1900), 20-keto-11-hydroxysugiol (m/z 329.1745), and 7-keto-carnosic acid (m/z 345.1695). c Enzyme activity summary of CYP76AKs.

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