Fig. 4: Chitin transporting channel of Chs1.
From: Structure, catalysis, chitin transport, and selective inhibition of chitin synthase
a Cut-in view of Chs1 (green) in complex with UDP-GlcNAc (orange), GlcNAc (purple), and Mg2+ (lime). Chs1 are shown in cartoon and elctrostatic surface. Two red arrows outline the path of chitin transporting channel. Chitin transporting channel of Chs1 in UDP-GlcNAc+GlcNAc bound state (b) and apo state (c). The channel was calculated by the program HOLE and illustrated by blue dots. The cytosolic gate and exoplasmic hydrophobic gate are highlighted by red arrows. d Pore sizes of the chitin transporting pathway in (b) and (c) are illustrated. e Putative transmembrane chitin-transporting channel of Chs1 with residues lining the channel shown as sticks. Labels of polar residue are highlighted in blue. Labels of residues forming the exoplasmic hydrophobic gate are highlighted by yellow background. f, g Two extremes of 3DVA analysis of the donor-bound Chs1 structure. The ___domain swapping loop, TMH4, and exoplasmic exit are labeled in two maps.
