Fig. 5: White matter astrocytes and gray matter astrocytes respond to injury differently and are slightly activated in the distal regions after SCI. | Nature Communications

Fig. 5: White matter astrocytes and gray matter astrocytes respond to injury differently and are slightly activated in the distal regions after SCI.

From: Single-cell analysis reveals region-heterogeneous responses in rhesus monkey spinal cord with complete injury

Fig. 5

a Violin plots showing the differentially expressed genes between white matter astrocytes (GM-AS) and gray matter astrocytes (WM-AS). b Immunostaining of SLC1A2, GFAP, and NeuN in the rhesus monkey spinal cord cross-section. Scale bars, 500 μm. c Immunostaining of GFAP and IL33 in the spinal cord cross-sections of rhesus monkey, human embryo (26 weeks), and mouse. Scale bars, 25 μm. d UMAP plot depicting the heterogeneity of astrocytes in the intact and injured spinal cord of rhesus monkey. WM-AS white matter astrocytes, AW-AS activated white matter astrocytes, GM-AS gray matter astrocytes, AG-AS activated gray matter astrocytes, D-AS dividing astrocytes. e Split UMAP plots showing the regional distribution of astrocyte subsets at distinct time points after SCI. f Gene expression visualized by UMAP. Each dot represents an individual cell colored according to the expression level. g Dynamic changes in the proportion of AW-AS and AG-AS in different regions after injury. h GO enrichment analysis of the upregulated genes of AW-AS and AG-AS compared with WM-AS and GM-AS, respectively. P values (adjusted) were calculated using Benjamini–Hochberg false discovery rate (FDR). i Violin plots showing the expression of injury-associated genes in astrocytes from SA and SL area. Source data are provided as a Source Data file.

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