Fig. 5: Circulating miR-204 alters mitochondria content in white adipose tissue.

a GSEA showing enrichment of genes related to indicated pathways (n = 3 mice for Control and n = 3 mice for 231/Ctrl mice). Nominal P values and FDR q values are shown. b Heat maps showing thermogenic gene expression in eWAT from mice receiving PBS and MDA-MB-231 sEVs. c, d The qRT-PCR analysis of mRNA abundance of Mt-Co1/Ndufv1 in iWAT and eWAT (n = 6 mice per group; one-way ANOVA followed by Dunnett multiple comparison test). e Representative transmission electron microscopy (TEM) images (left), quantification of lipid droplet size and mitochondrial content (right) in differentiated primary adipocytes from iWAT following sEVs treatment (n = 3 random fields per group; one-way ANOVA followed by Dunnett multiple comparison test). Representative H&E staining with iWAT and eWAT in mice from xenograft tumour model f and mice receiving sEVs injection model g. Repeated three times independently with similar results obtained. Scale bar, 200 μm. Representative immunohistochemistry staining of UCP1 in iWAT and eWAT from both xenograft tumour model h and mice receiving sEV injection model. i Repeated three times independently with similar results obtained. Scale bar, 200 μm. Data are presented as mean ± s.e.m; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. Source data and exact P value are provided as a Source data file.