Fig. 7: Exosomal miR-204 promotes thermogenesis and lipolysis in C26-xenografted tumour mice.

a The expression level of miR-204 in C26 and C26/miR-204 KO derived sEVs (n = 3 biologically independent samples per group; unpaired two-tailed t-test). b Body weight (calculated by substracting tumour weight from the total weight) of mice bearing C26 tumours (n = 7 mice per group; one-way ANOVA followed by Dunnett multiple comparison test). c Relative tissues weight included iWAT, epididymal white adipose tissue (eWAT), BAT, GA, TA (normalized to body weight) in tumour-free, C26/Ctrl and C26/miR-204 KO group mice (n = 7 mice per group; one-way ANOVA followed by Dunnett multiple comparison test). d Daily food intake (n = 8 mice per group; one-way ANOVA followed by Dunnett multiple comparison test). e Rectal temperatures of the mice at different times after cold exposure (n = 5 mice per group; one-way ANOVA followed by Dunnett multiple comparison test). f The oxygen consumption (VO₂) and g Heat production from indicated groups. Dot plots represent the 48 h average values (n = 6 mice per group; one-way ANOVA followed by Dunnett multiple comparison test). h The qRT-PCR analysis of miR-204 abundance of serum sEVs from each group mice (n = 5 mice per group; one-way ANOVA followed by Dunnett multiple comparison test). i, j Relative mRNA abundance of the indicated thermogenic markers in iWAT and BAT from each group mice (n = 5 mice per group; one-way ANOVA followed by Dunnett multiple comparison test). k Western blots showing indicated proteins in iWAT and eWAT from each group mice. (tumour free group, n = 4 mice per group; C26/Ctrl group, n = 4 mice per group; C26/miR-204 KO group, n = 5 mice per group). l Image of excised tumours (n = 4 mice per group). m Tumour volumes were measured every 3 days (n = 7 mice per group; unpaired two-tailed t-test) and (n) tumour weight when mice were sacrificed (n = 7 mice per group; unpaired two-tailed t-test). o Representative H&E staining with iWAT/eWAT from each group mice. Repeated three times independently with similar results obtained. Scale bar, 200  μm. p Representative immunohistochemistry staining of HIF1Α in iWAT and eWAT. Repeated three times independently with similar results obtained. Scale bar, 200 μm. q Representative immunohistochemistry staining of UCP1 in iWAT and eWAT. Repeated three times independently with similar results obtained. Scale bar, 200 μm. Data are presented as mean±s.e.m; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns: not significant. Source data and exact P value are provided as a Source data file.