Fig. 2: CAST regulation by two conformational regulators, T1 and T2. | Nature Communications

Fig. 2: CAST regulation by two conformational regulators, T1 and T2.

From: Programming conformational cooperativity to regulate allosteric protein-oligonucleotide signal transduction

Fig. 2

a Schematic illustration of the CAST system. b Detailed design of regulators T1 and T2. c Fluorescence assay of the CAST regulation after altering the length combinations of T1 and T2 as follows (T1 length, T2 length): (I) 3 nt, 4 nt; (II) 3 nt, 8 nt; (III) 3 nt, 12 nt; (IV) 16 nt, 12 nt; (V) 25 nt, 12 nt. In each case, the concentration of DNA complex CDAB was 0.6 µM, while the concentrations of thrombin were 0, 0.3, 0.9, and 1.5 µM. d The down-regulation and up-regulation CAST effects of the individual regulators T1 and T2. e Histograms of the fluorescence data of the CAST regulation with varying T1 and T2 lengths and a range of thrombin concentrations: 0, 0.3, 0.9, and 1.5 µM.

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