Fig. 3: Two-input logic operations based on the CAST strategy. | Nature Communications

Fig. 3: Two-input logic operations based on the CAST strategy.

From: Programming conformational cooperativity to regulate allosteric protein-oligonucleotide signal transduction

Fig. 3

a, b Schematic illustration of an OR logic gate. c PAGE results of OR logic operation using DNA complex-or (CDORA1B1). Lane 1: DOR; Lane 2: CA1B1; Lane 3: CA1B1 + Thr; Lane 4: CA1B1 + SA; Lane 5: complex-or; Lane 6: complex-or + Thr; Lane 7: complex-or + SA; Lane 8: complex-or + Thr + SA. d Fluorescence assay of OR logic operation. e, f Schematic illustration of AND logic gate. g PAGE results of AND logic operation using DNA complex-and (C1B2 + C2A2DAND). Lane 1: DAND; Lane 2: C1B2 + C2A2; Lane 3: C1B2 + C2A2 + Thr; Lane 4: C1B2 + C2A2 + SA; Lane 5: C1B2 + C2A2 + Thr + SA; Lane 6: complex-and; Lane 7: complex-and + Thr; Lane 8: complex-and + SA; Lane 9: complex-and + Thr + SA. h Fluorescence assay of AND logic operation. [DNA strands] = 0.6 µM, [Thr] = 1.5 µM, [SA] = 1.8 µM. i Cascading CAST circuit triggered by thrombin and streptavidin. j The illustrations and k designs of cascading CAST logic operation (using DNA complex (XI), ZS*XY and complex (XII), ODCdR*S), respectively. S* and DCd are the upstream and downstream outputs of the cascade circuit, respectively. l Fluorescence results. [complex (XI)] = 0.5 μM, [complex (XII)] = 0.5 μM, [Thr] = 1.25 μM, [SA] = 1.5 μM.

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